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Antibodies bound to the blocking peptide now not bind for the epitope on the focus on protein. By comparing the staining within the blocked antibody compared to the antibody by itself, one can see which staining is specific.

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This guidebook outlines further more achievable optimization of mobile and immunoassay parameters to make sure the very best results are...

This information outlines further more feasible optimization of mobile and immunoassay parameters to be certain the best possible benefits are...

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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Clicking the pictures or hyperlinks will redirect you to definitely a website hosted by BenchSci that provides 3rd-celebration scientific material. Neither the information nor the BenchSci technologies and procedures for collection happen to be evaluated by us; we have been furnishing them as-is and without having guarantee of any kind, which includes to be used or software from the Thermo Fisher Scientific merchandise introduced.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...

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Blocking peptides are peptides that bind particularly towards the target antibody and block antibody binding. These peptide generally consists of the epitope recognized from the antibody.

Target Information and facts Cyclin E belongs into the really conserved cyclin relatives, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S section.

Overexpression of the gene has been noticed in several tumors, which ends up in chromosome instability, and so may well lead to tumorigenesis. This protein was found to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important part in endorsing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are described. Two further splice variants were documented but in-depth nucleotide sequence info is not really still obtainable.

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